The music streaming firm is the latest to save on costs as consumers are squeezed by high living costs and businesses shy away from advertising.
Spotify has become the latest company in the technology sphere to announce big job cuts, with 6% of the workforce to go in the coming months.
The Sweden-based US-listed music streaming firm said a broader shake-up of its operations would see its chief content officer Dawn Ostroff leave the business.
The number of employees set to depart would total around 600 people - based on the company's last official count.
Spotify said it was to take a severance-related charge of between €35m (£30.7m and €45m (£39.6m).
Shares rose by more than 4% in pre-market trading.
It is the latest household name to cut large numbers of jobs to try and save cash as the global economy remains under intense pressure from the fallout from Russia's war in Ukraine.
Daniel Ek is the co-founder and chief executive of Spotify
Fears remain of a recession in the world's largest economy.
Companies such as Spotify, Google parent firm Alphabet and Facebook's Meta have each reported a slowdown in key advertising revenues.
Spotify had said in October that it would slow down hiring for the rest of the year and into 2023.
Microsoft was the latest to reveal major lay-offs last week, totalling 10,000 people.